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Korean J Crit Care Med > Volume 13(2); 1998 > Article
Korean Journal of Critical Care Medicine 1998;13(2): 194-197.
뇌교종세포에서 Thiopental 투여에 따른 등장액에서의 세포용적 변화
이영석, 문봉기, 한상건, 이영주, 홍정연, 이경진, 윤수한1, 정금회2, 길현주2
아주대학교 의과대학 마취과학교실 ,1신경외과학교실 , 2포천 의과대학 마취과학교실
Volume Changes under Isotonic Condition Containing Thiopental in Brain Astrocytoma Cells
Young Seok Lee, Bong Ki Moon, Sang Gun Han, Young Joo Lee, Jeong Yeon Hong, Kyeong Jin Lee, Soo Han Yoon, Keum Hee Chung, Hyun Jue Gill
1Department of Anesthesiology, Ajou University School of Medicine, Suwon, Korea.
2Department of Neurosurgery, Ajou University School of Medicine, Suwon, Korea.
3Department of Anesthesiology, Pochun Medical College, Sungnam, Korea.
BACKGOUND: Cell volume regulation is especially important in the brain because the brain is confined within a non-compliant vault and cannot tolerate significant perturbations in cell size. Cerebral cell volume regulation mechanisms are activated by sustained disturbances in plasma osmolality. The constancy of cell volume under physiological conditions is generally thought to reflex a balance between influx and efflux of solute and is therefore critically dependent on the properties of the plasma membrane. Cell volume regulation have not been described under isoosmotic solution. The object of the study was to know the effects of thiopental on cell volume change in isoosmotic condition. METHODS: We made isoosmotic solution without thiopental (Group 1) and isoosmotic solution with 22.9 mM (Group 2), 16.8 mM (Group 3), 13.3 mM (Group 4) thiopental, separately, in order to study changes in cell volume under isoosmotic solution. We put cultured human brain astrocytoma cells into isoosmotic solution for each group and calculated cell volume using Coulter Counter after 30 minutes. RESULTS: Cell volume was shown to be 5084+/-8580 (micrometer3)in Group 1, 501+/-854 (micrometer3) in Group 2, 1183+/-3839 (micrometer3) in Group 3, and 624+/-1100 (micrometer3) in Group 4. We discovered that cells in Group 2,3,4 were shrunk relative to cells in Group 1 (p<0.01). And there were significant differences in cell volume among thiopental groups. CONCLUSIONS: Thiopental may has an effect on cell membrane properties and decrease cell volume under isoosmotic solution in brain astrocytoma cell.
Key Words: Anesthetic; intravenous; thiopental; Brain; astrocytoma; volume regulation; isoosmotic solution
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