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Korean J Crit Care Med > Volume 25(3); 2010 > Article
Korean Journal of Critical Care Medicine 2010;25(3): 149-154. doi: https://doi.org/10.4266/kjccm.2010.25.3.149
이트라코나졸 전처치가 내독소로 유도된 백서의 급성 폐손상에 미치는 영향
*한림대학교 의과대학 내과학교실, †대구가톨릭대학교 의과대학 생리학교실, ‡울산대학교 의과대학 서울아산병원 내과학교실
The Effect of Itraconazole Pretreatment in Lipopolysaccharide-Induced Acute Lung Injury in Rats
Tae Rim Shin, Young Man Lee, Younsuck Koh
1Department of Internal Medicine, Hallym University College of Medicine, Chuncheon, Korea.
2Department of Physiology, Catholic University of Daegu College of Medicine, Daegu, Korea.
3Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. yskoh@amc.seoul.kr
BACKGROUND: Despite the fact that a randomized controlled trial did not support the use of ketoconazole for treatment of acute lung injury (ALI), there is evidence that pretreatment with ketoconazole might prevent ALI in critically ill patients. An in vitro study showed, however, that itraconazole was a more potent inhibitor of thromboxane and leukotriene formation than was ketoconazole. We investigated the effect of itraconazole pretreatment in lipopolysaccharide (LPS)-induced ALI in rats. METHODS: Twenty-one pathogen free, male Sprague-Dawley rats were administered either saline or LPS (5 mg/kg of body weight) intratracheally, with or without intraperitoneal pretreatment of itraconazole (2.5 mg/kg). Six hours after saline or LPS treatment (7 h after itraconazole pretreatment), samples were obtained. RESULTS: Compared with the saline group, LPS group had increased total cell count, polymorphonuclear leukocyte differential count, protein, lactate dehydrogenase (LDH) and cytokines in BAL fluid. Itraconazole pretreatment decreased polymrphonuclear leukocyte differential count, protein and LDH in BAL fluid compared with those of LPS-treated rats without itraconazole pretreatment. Itraconazole pretreatment also decreased the elevated BAL fluid levels of interleukin-1beta (IL-1beta) and cytokine-induced neutrophil chemoattractant (CINC) by LPS. There was, however, no difference in the BAL fluid tumor necrosis factor alpha (TNF-alpha) level in terms of itraconazole pretreatment in LPS-treated rats. Histopathologic features of LPS-induced ALI were attenuated by itraconazole pretreatment. CONCLUSIONS: These results suggest that itraconazole pretreatment attenuated LPS-induced ALI in rats. Decreases in levels of IL-1beta and CINC would likely be associated with attenuation of LPS-induced ALI in rats by itraconazole pretreatment.
Key Words: acute lung injury; cytokine; itraconazole; lipopolysaccharide
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