Skip Navigation
Skip to contents

ACC : Acute and Critical Care

OPEN ACCESS
SEARCH
Search

Articles

Page Path
HOME > Acute Crit Care > Volume 26(3); 2011 > Article
Original Article Effect of Caffeic Acid Phenethyl Ester on Lipopolysaccharide-induced Murine Macrophage Activation
Seong Heon Lee, Mei Li, Dae Wook Lee, Dong Yun Lim, Cheol Won Jeong, Sang Hyun Kwak

DOI: https://doi.org/10.4266/kjccm.2011.26.3.134
  • 2,719 Views
  • 28 Download
  • 0 Crossref
  • 0 Scopus
1Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, Gwangju, Korea. shkwak@jnu.ac.kr
2Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

BACKGROUND
Caffeic acid phenethyl ester (CAPE) is an active component of propolis and is known to have anti-inflammatory properties. This study was performed to evaluate the effects of CAPE on lipopolysaccharide (LPS)-induced murine macrophage activation.
METHODS
Raw 264.7 cells were incubated with varying concentrations of CAPE with or without LPS. The production of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta and macrophage inflammatory protein-2 (MIP-2) and activation of extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 were measured.
RESULTS
CAPE inhibited the production of TNF-alpha, IL-1beta and MIP-2 and attenuated phosphorylation levels of ERK1/2 and p38, but not JNK in RAW264.7 cells stimulated with LPS.
CONCLUSIONS
CAPE can attenuate LPS-induced macrophage responses and we suggest that these effects may play an important role in modulating macrophage-mediated inflammatory responses in vivo.


ACC : Acute and Critical Care