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HOME > Acute Crit Care > Volume 38(3); 2023 > Article
Original Article
Cardiology A comparative study of stored arterial versus venous blood collected using the acute normovolemic hemodilution method in coronary artery bypass grafting patients in Iran
Mojtaba Mansouri1orcid, Alireza Yazdani1orcid, Gholamreza Masoumi1orcid, Mohsen Mirmohammadsadeghi2orcid, Amir Mirmohammadsadeghi2orcid
Acute and Critical Care 2023;38(3):353-361.
DOI: https://doi.org/10.4266/acc.2022.01382
Published online: June 15, 2023

1Department of Anesthesiology, Anesthesiology Research Center, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2Department of Surgery, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Corresponding author: Alireza Yazdani Department of Anesthesiology, School of Medicine, Isfahan University of Medical Sciences, Hezarjerib Ave, Isfahan 8174673461, Iran Tel: +98-31-3792-3243, Fax: +98-31-3668-8628, Email: dr_alirezayazdani@yahoo.com
• Received: November 1, 2022   • Revised: March 16, 2023   • Accepted: March 19, 2023

Copyright © 2023 The Korean Society of Critical Care Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Background
    In the present study, arterial and venous blood was collected from patients who were candidates for elective coronary artery bypass grafting (CABG); the blood was stored for 28 days and cellular, biomechanical, and hematological changes in blood were compared to determine whether stored arterial blood is superior to stored venous blood.
  • Methods
    The present follow-up comparative study included 60 patients >18 years of age, with hemoglobin >14 mg/dl and ejection fraction >40% who were candidates for CABG. After induction of anesthesia, 250 ml of arterial or venous blood was drawn from patients (arterial blood group and venous blood group). Laboratory blood samples were taken at specified times from the collected blood and re-injected into the patients after CABG.
  • Results
    Significant differences were observed in pH, partial pressure of carbon dioxide (PCO2), partial pressure of oxygen (PO2), bicarbonate (HCO3), and glucose values at several time points between the groups. Other parameters such as urea and creatinine did not show any significant differences between the groups.
  • Conclusions
    Twenty-eight days of storage can have a negative effect on some of the cellular, biochemical, and hematological components of arterial and venous blood; however, the quality of stored arterial blood and venous blood does not differ significantly.
  • Key Words: Hemodilution; coronary artery bypass graft; stored blood
Transfusion of stored blood is a common medical intervention intended to improve oxygen delivery to body tissues and reduce the complications of anemia or related medical procedures [1]. Blood is routinely stored at 2 °C–6 °C for up to 35 days in preservatives such as citrate phosphate dextrose adenine (CPDA). During storage, several physical, biochemical, and biomechanical changes occur in blood products (storage lesion) that reduce their survival and function [2]. The changes include prominent alterations in red blood cell (RBC) morphology known as red cell storage lesion and blood stored for a long period of time is more affected than when stored for a short period [3]. Storage lesion leads to reduced adenosine triphosphate (ATP) and hemoglobin (Hb) level, increased lactate level with resulting lower pH, increased lysis, minimized blood movement and oxygen transport, decreased intracellular potassium (K), and increased sodium (Na) in the cytoplasm [3-5]. Consequently, red cell storage lesion reduces efficacy of the transfused blood products which increases the chances of transfusion-related morbidities [5].
In a previous study, the extent of correlation between arterial and peripheral venous samples for blood gases and acid base status in critically ill and emergency department patients were investigated. The results showed that venous blood may be a useful alternative to arterial blood during blood gas analysis, obviating the need for arterial puncture in difficult clinical situations, especially in trauma patients, for initial emergency department assessment and early stages of resuscitation [6].
In another investigation, significant changes were observed in some biochemical parameters such as serum aspartate aminotransferase, total protein, albumin, urea, chloride, and K levels assessed at periodic intervals. These changes might be caused by spontaneous hemolysis or non-viable RBCs due to storage, thus, blood should be transfused as early as possible to prevent a negative effect on the biochemical composition of RBCs [7].
However, less evidence exists regarding changes in donated arterial and venous blood after storage. Therefore, assessing the difference in donated arterial and venous blood after storage is beneficial for managing patients in emergency conditions.
In the present study, the cellular, biochemical, and hematological changes in donated arterial and venous blood after 28 days of storage were compared in patients who were candidates for elective coronary artery bypass grafting (CABG).
The present follow-up comparative study was conducted in hamran heart hospital in 2021 in Iran. Sixty patients >18 years of age with Hb >14 mg/dL and ejection fraction (EF) >40% scheduled for elective CABG were enrolled in the study. Subjects were selected using convenience sampling method. Sampling was continued until the required number of samples was obtained. Patients with emergency surgery, severe renal and hepatic dysfunction, hematological disorders, EF <40%, or undergoing a reoperation were excluded. The Ethics Committee of Isfahan university of medical science approved this study (ethics code IR.MUI.MED.REC.1401.114). In addition, written informed consent was obtained from all participants.
First, patients were monitored using a five-lead electrocardiogram, pulse oximetry, noninvasive blood pressure monitoring, and capnography, and their radial artery was cannulated for blood sampling. All patients were anesthetized in the same manner; general anesthesia was administered with fentanyl (5 µg/kg) as a narcotic drug, cisatracurium (0.2 mg/kg) as a muscle relaxant, and Na thiopental (5 mg/kg) as a hypnotic drug. Then, tracheal intubation was performed and the lungs were ventilated using a ventilator adjusted to produce an arterial partial pressure of carbon dioxide (PCO2) of 35–45 mm Hg. Anesthesia was maintained with propofol (100 µg/kg/min) and morphine (0.2 mg/kg). A central venous catheter was inserted into the right internal jugular vein in all patients after anesthesia induction. Patients were randomly assigned to two groups based on arterial and venous blood donation. Donated arterial blood was collected from the radial artery in patients in the arterial group and donated venous blood was obtained from the peripheral vein in patients in the venous group. Blood was slowly drawn into bags containing 50 mL CPDA-1 (Figure 1).
Acute normovolemic hemodilution, which is essential to minimize surgical blood loss, was performed immediately after induction of anesthesia and before heparin injection and starting the cardiopulmonary pump. The patient had 250 ml of arterial or venous blood drawn and simultaneously replaced with a triple volume of crystalloid (750 ml intravenous saline or Ringer's solution) through the central or peripheral venous cannula to maintain normovolemia.
The first blood sample was then sent to the laboratory for venous blood gas and arterial blood gas analyses, complete blood count, and biochemistry (creatinine [Cr], lactate dehydrogenase (LDH), albumin, Na, K, platelet, glucose, mean corpuscular hemoglobin [MCH], mean corpuscular volume [MCV], and MCH concentration [MCHC]) analysis.
The collected blood was re-injected into the patients through peripheral venous cannula after CABG and hemostasis established and protamine injected. During the blood transfusion, the second blood sample was collected from the patients and immediately sent to the hospital laboratory for analysis.
After the blood infusion, 10 ml of the blood remained in the bag, which was immediately transferred to the hospital laboratory. This sample was stored in the blood bank refrigerator at a temperature between 1 °C –6 °C for 28 days and then analyzed.
Statistical Analysis
The results are expressed as the mean± standard deviation. The Student t-test was used to compare parametric variables and the chi-square test to compare categorical variables between groups. Time course experiments of blood were analyzed using repeated measures analysis of variance with post-hoc Bonferroni for multiple comparisons at each time point. A P-value <0.05 was considered to indicate statistical significance. All the statistical analyses were performed using IBM SPSS ver. 21 (IBM Corp.).
In the present study, 60 patients were divided into two groups using convenience sampling: 30 in the arterial blood group and 30 in the venous blood group (Figure 1). Sampling was continued until the required number of samples was obtained. Table 1 shows that all demographic data were similar between the groups with the exception of age (P=0.003).
The results showed the changes in donated blood at three time points were significantly different in almost all parameters in both groups except hematocrit (P=0.445 in arterial group and P=0.122 in venous group) and MCH (P=0.476 in arterial group and P=0.109 in venous group). In addition, the changes in albumin were not significant in the arterial group (P=0.363) (Table 2). P-values for intervention showed the changes between the groups were not significantly different (Table 2).
As shown in Table 2, PCO2 in the venous blood group was significantly higher than in the arterial blood group immediately after donating blood (57.25±10.63 vs. 49.60±8.33) and 4 hours after donating blood (62.23±7.61 vs. 54.81±7.79). However, after 28 days of storage, significant difference was not observed in PCO2 between the groups (P=0.883).
In addition, partial pressure of oxygen (PO2) in the arterial blood group was significantly higher than in the venous blood group immediately after donating blood (190.82±124.57 vs. 68.94±18.62) and 4 hours after donating blood (131.60±61.84 vs. 71.38±20.31). After 28 days of storage, significant difference was not observed in PO2 between the groups (P=0.568). Significant differences were observed in pH (P=0.02) and bicarbonate (HCO3; P=0.021) between the groups 4 hours after donating blood. Immediately after donating blood, significant difference was observed in glucose (P=0.007) which was significantly lower in the arterial blood group than in the venous blood group (431.53±132.08 vs. 513.67±90.18) before CABG (Table 2). P-values for interaction showed no interaction between the groups in all parameters except PO2 (P=0.03) and platelets (P=0.02) (Table 2).
Anemia affects one-quarter of the global population, nearly 50% of hospitalized patients, and up to 75% of hospitalized elderly patients. A high percentage of patients (60%–66%) become anemic during intensive care unit stay. Anemia is associated with a range of postoperative consequences, major side effects, rehospitalization, duration of stay in the intensive care unit, hospital stay, and mortality [8]. Therefore, blood transfusion is a vital and life-saving measure in many emergency situations [9].
In the present study, parameters such as albumin, LDH, Cr, urea, platelets, MCHC, MCH, Hb, white blood cells (WBCs), RBCs, lactate, K, Na, MCV, and hematocrit did not show significant differences at any time point between the groups. However, arterial pH and HCO3 were higher than venous pH and HCO3 after CABG. Arterial PCO2 was lower than venous PCO2 before and after CABG. Arterial PO2 was significantly higher than venous PO2 before and after CABG. In addition, arterial glucose was lower than venous glucose before CABG. The venous blood will have a lower PO2, lower pH, and higher PCO2 than the arterial blood due to metabolism in the tissues.
The results of the present study showed the mean values of arterial Na and K were lower than venous Na and K but without statistical significance; however, these differences were significant in another study [10]. Those differences were attributed to the effect of heparin because arterial samples were collected in heparinized containers [10].
The present findings showed the changes in arterial and venous RBCs, WBCs, Hb, MCV, MCHC, and platelets were significantly different after 28 days of storage. The WBCs significantly decreased in both groups after 28 days of storage. Similarly, in previous studies, significant reduction in WBCs was observed after 35 days of storage [11,12]. In addition, WBCs were shown to constantly decrease during the 28-day storage period [13].
The results of the present study showed that MCV significantly increased in both groups, which is in agreement with previous studies in which MCV was significantly increased during storage [11,14]. The RBCs, Hb, MCHC, and platelets significantly decreased after 28 days of storage. However, albumin showed significant decrease only in the venous group after 28 days of storage.
The decreased levels might be due to hemolysis that occurs during storage. In a previous study, significant reduction was observed in RBCs at day 28 and further significantly decreased during 35 days of storage which was in agreement with our finding [11]. However, the results of the present study are in contrast to a previous study in which statistically significant changes were not observed in RBCs during the 28-day storage period [15]. During blood storage at 2 °C–6 °C, glycolysis is reduced and the amount of ATP is also reduced, thus, the structure and volume of the RBCs are changed [16].
In a previous study, Hb concentration gradually decreased during the 35-day storage period, which is in agreement with our findings and might be due to hemolysis that occurs during storage [14]. Significant reduction in MCHC was found in a previous study, similar to the results of the present study [11]. As reported in previous studies, the changes in MCH and MCHC can be the result of a deregulated mechanism of cell volume, which causes the increase in the volume of RBCs, increasing hypochromia and anisocytosis. Therefore, the decrease in MCHC apparently is the result of an increase in cell volume [17].
In the present study, platelets were significantly decreased after 28 days of storage. This finding was in agreement with Marabi et al. [11], and can be explained by the cells losing viability due to ATP depletion in addition to platelet utilization caused by micro-aggregate development [18].
Contradictory results have been reported for hematocrit. A slight decrease in hematocrit was found in both groups in the present study but without statistical significance. In a previous study, significant increase in hematocrit was observed at day 35 of storage [11], however, this finding was refuted in another study [14]. Decrease in hematocrit during storage can be attributed to depletion of RBC ATP or morphological alterations that occur during blood storage [14,19].
In addition, MCH changes were insignificant in both groups which is in agreement with a study in which the MCH changes were insignificant during 28 days of storage [15]. However, MCH reportedly decreased during the storage period in another study [20].
In the present study, significant increase in lactate was observed after 28 days of storage in both groups. Whole blood stored for more than 14 days was proposed to have reduced efficacy with increased markers of red cell storage lesion, such as increased K level, lactate, and decreased pH [1]. Based on previous studies, a decrease in pH is the direct result of the increase in lactate level due to anaerobic metabolism of glucose [21-23].
In the present study, the changes in Na and K were significantly different in each group. The results showed significant decrease in Na and significant increase in K in both groups after 28 days of storage. In agreement with our results, Antwi-Baffour et al. [24] demonstrated that K concentration in refrigerated blood increases and plasma Na concentration decreases with storage time.
The present findings indicated a significant reduction in glucose and HCO3 after 28 days of storage. However, other parameters such as LDH, urea, and Cr significantly increased in both groups after 28 days of storage.
A significant increase in K concentration and decrease in HCO3 levels from 1 day to 21 days of storage were reported in another study which is in agreement with the results of the present study [25].
In other studies, decreased concentration of plasma glucose and HCO3 and increase in K, lactate, and LDH concentration with storage were observed which confirms the results of the present study [26].
Contrary to the present study, Verma et al. [25] reported no significant changes in urea and Cr during 21 days of whole blood storage.
The glucose concentration is limited and glucose is used during storage, thus, ATP depletion and decrease in red cell viability occur concomitantly. Therefore, the energy required for operating the ATPase pump in RBCs decreases over time. Inhibition of the Na pump results in hyperkalemia and hyponatremia as observed in the present study [25].
As a result of glycolysis, the concentration of lactic acid increases which may cause a decrease in pH, subsequently significantly increasing LDH. In addition, hemolysis results in the increased concentration of LDH and the decreased concentration of RBCs, Hb, MCHC, and platelets in plasma [1,25].
Based on the results, although significant biochemical changes occurred in the donated arterial and venous blood groups during the blood storage period, the process of biochemical changes between the two groups did not show any significant difference. Therefore, arterial blood donation for blood banking cannot be recommended over venous blood donation.
▪ Significant changes were not found in urea and creatinine at 28 days of whole blood storage.
▪ Blood should be transfused as early as possible to prevent a negative effect on the cellular, biochemical, and hematological components of blood.
▪ After candidates for elective coronary artery bypass grafting, significant differences in pH and bicarbonate (HCO3) were observed between the arterial and venous blood groups.

CONFLICT OF INTEREST

No potential conflict of interest relevant to this article was reported.

FUNDING

None.

AUTHOR CONTRIBUTIONS

Conceptualization: AY. Data curation: AY. Formal analysis: GM. Funding acquisition: MM. Methodology: AY, MM. Project administration: MM. Visualization: AY, AM. Writing–original draft: AY, MM. Writing–review & editing: AY, GM, MM.

None.
Figure 1.
CONSORT flow diagram.
acc-2022-01382f1.jpg
Table 1.
Comparison of demographic variables between groups
Variable Arterial blood Venous blood P-value
Age (yr) 64.33±9.19 55.43±12.65 0.003
Sex 0.125
 Male 24 (80.0) 28 (93.3)
 Female 6 (20.0) 2 (6.7)
Diabetes 0.781
 Yes 10 (33.3) 9 (30.0)
 No 20 (66.7) 21 (70.0)
Smoking 0.513
 Yes 5 (16.7) 7 (23.3)
 No 25 (83.3) 23 (76.7)

Values are presented as mean±standard deviation or number (%).

Table 2.
Comparison between and within blood groups
Variable Time Arterial blood Venous blood P-value
pH Immediately after donation 7.15±0.11 6.09±0.10 0.05
4 Hours after donation 7.07±0.06 7.04±0.06 0.02
28 Days after donation 6.59±0.34 6.45±0.24 0.06
P-value <0.001 <0.001
P-value for interaction 0.804
P-value for intervention 0.288
PCO2 Immediately after donation 49.60±8.33 57.25±10.63 0.003
4 Hours after donation 54.81±7.79 62.23±7.61 0.001
28 Days after donation 27.31±11.19 27.72±9.8 0.883
P-value <0.001 <0.001
P-value for interaction 0.594
P-value for intervention 0.239
pO2 Immediately after donation 190.82±124.57 68.94±18.62 <0.001
4 Hours after donation 131.60±61.84 71.38±20.31 <0.001
28 Days after donation 237.82±36.90 243.09±31.10 0.568
P-value <0.001 <0.001
P-value for interaction 0.033
P-value for intervention 0.393
HCO3 Immediately after donation 16.83±2.84 17.01±2.21 0.785
4 Hours after donation 15.40±1.66 16.34±1.38 0.021
28 Days after donation 2.09±1.74 1.98±1.66 0.800
P-value <0.001 <0.001
P-value for interaction 0.500
P-value for intervention 0.878
Na Immediately after donation 146.75±5.16 148.51±3.66 0.135
4 Hours after donation 149.52±4.03 150.84±4.00 0.210
28 Days after donation 140.14±6.75 142.77±7.75 0.168
P-value <0.001 <0.001
P-value for interaction 0.153
P-value for intervention 0.102
K Immediately after donation 3.22±1.76 2.85±0.50 0.269
4 Hours after donation 2.63±0.46 2.73±0.59 0.494
28 Days after donation 17.23±2.39 18.06±2.31 0.178
p-value <0.001 <0.001
p-value for interaction 0.352
p-value for intervention 0.281
Glucose Immediately after donation 431.53±132.08 513.67±90.18 0.007
4 Hours after donation 416.63±123.37 446.17±123.33 0.602
28 Days after donation 223.63±68.40 235.43±60.97 0.538
p-value <0.001 <0.001
p-value for interaction 0.503
p-value for intervention 0.509
Lactate Immediately after donation 11.39±3.66 12.46±3.05 0.224
4 Hours after donation 21.44±4.29 19.34±4.14 0.059
28 Days after donation 175.03±15.26 173.40±13.73 0.665
P-value <0.001 <0.001
P-value for interaction 0.364
P-value for intervention 0.543
RBC Immediately after donation 3.92±0.53 4.11±0.45 0.143
4 Hours after donation 3.95±0.75 3.95±0.95 0.986
28 Days after donation 3.24±0.77 3.47±0.69 0.233
Immediately after donation <0.001 <0.001
P-value for interaction 0.210
P-value for intervention 0.448
WBC Immediately after donation 5.45±1.39 5.31±1.06 0.643
4 Hours after donation 5.24±1.40 5.74±1.53 0.189
28 Days after donation 4.69±1.48 4.84±1.24 0.665
P-value 0.001 0.014
P-value for interaction 0.095
P-value for intervention 0.783
Hemoglobin Immediately after donation 12.12±1.66 12.52±1.50 0.327
4 Hours after donation 11.99±2.13 11.83±2.11 0.762
28 Days after donation 9.96±2.39 10.30±2.20 0.569
Immediately after donation <0.001 <0.001
P-value for interaction 0.703
P-value for intervention 0.867
Hematocrit Immediately after donation 35.82±4.50 37.84±3.31 0.055
4 Hours after donation 35.89±6.17 35.67±5.47 0.886
28 Days after donation 34.61±7.82 35.95±7.30 0.497
Immediately after donation 0.445 0.122
P-value for interaction 0.413
P-value for intervention 0.394
MCV Before CABG 90.67±3.96 92.16±4.87 0.196
End of CABG 90.12±6.53 91.58±4.44 0.313
28 Days after CABG 103.14±10.21 104.15±11.66 0.722
P-value <0.001 <0.001
P-value for interaction 0.105
P-value for intervention 0.775
MCH Immediately after donation 30.79±1.79 30.75±1.88 0.926
4 hours after donation 31.05±1.71 31.03±2.15 0.971
28 Days after donation 30.88±1.84 30.67±1.71 0.651
P-value 0.476 0.109
P-value for interaction 0.650
P-value for intervention 0.305
MCHC Immediately after donation 33.74±2.03 33.53±1.89 0.679
4 Hours after donation 33.85±1.88 33.94±1.90 0.848
28 Days after donation 29.85±2.27 29.74±2.67 0.864
P-value <0.001 <0.001
P-value for interaction 0.211
P-value for intervention 0.388
Platelet Immediately after donation 190.30±45.67 170.37±41.11 0.081
4 Hours after donation 171.67±40.42 158.07±39.57 0.193
28 Days after donation 154.17±89.87 139.17±53.04 0.434
P-value 0.045 0.004
P-value for interaction 0.022
P-value for intervention 0.326
Urea Immediately after donation 31.50±8.71 32.51±9.60 0.672
4 Hours after donation 32.71±9.38 32.61±9.02 0.968
28 Days after donation 36.73±11.22 37.48±9.92 0.786
P-value <0.001
P-value for interaction 0.523
P-value for intervention 0.192
Creatinine Immediately after donation 1.03±0.20 1.10±0.21 0.232
4 Hours after donation 1.09±0.18 1.16±0.22 0.186
28 Days after donation 1.18±0.27 1.22±0.28 0.623
P-value <0.001 <0.001
P-value for interaction 0.594
P-value for intervention 0.092
LDH Immediately after donation 228.20±70.37 245.60±68.50 0.336
4 Hours after donation 240.23±95.93 259.07±92.10 0.441
28 Days after donation 1,306.37±352.06 1,230.08±396.83 0.434
P-value <0.001 <0.001
P-value for interaction 0.133
P-value for intervention 0.818
Immediately after donation Immediately after donation 3.33±0.56 3.51±0.46 0.170
4 Hours after donation 3.23±0.52 3.36±0.56 0.350
28 Days after donation 3.32±0.45 3.48±0.44 0.147
P-value 0.363 0.010
P-value for interaction 0.579
P-value for intervention 0.443

PCO2: partial pressure of carbon dioxide; PO2: partial pressure of oxygen; HCO3: bicarbonate; Na: sodium; K: potassium; RBC: red blood cell; WBC: white blood cell; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; LDH: lactate dehydrogenase.

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        A comparative study of stored arterial versus venous blood collected using the acute normovolemic hemodilution method in coronary artery bypass grafting patients in Iran
        Acute Crit Care. 2023;38(3):353-361.   Published online June 15, 2023
        DOI: https://doi.org/10.4266/acc.2022.01382
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      A comparative study of stored arterial versus venous blood collected using the acute normovolemic hemodilution method in coronary artery bypass grafting patients in Iran
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      Figure 1. CONSORT flow diagram.

      Figure 1.

      A comparative study of stored arterial versus venous blood collected using the acute normovolemic hemodilution method in coronary artery bypass grafting patients in Iran
      Variable Arterial blood Venous blood P-value
      Age (yr) 64.33±9.19 55.43±12.65 0.003
      Sex 0.125
       Male 24 (80.0) 28 (93.3)
       Female 6 (20.0) 2 (6.7)
      Diabetes 0.781
       Yes 10 (33.3) 9 (30.0)
       No 20 (66.7) 21 (70.0)
      Smoking 0.513
       Yes 5 (16.7) 7 (23.3)
       No 25 (83.3) 23 (76.7)
      Variable Time Arterial blood Venous blood P-value
      pH Immediately after donation 7.15±0.11 6.09±0.10 0.05
      4 Hours after donation 7.07±0.06 7.04±0.06 0.02
      28 Days after donation 6.59±0.34 6.45±0.24 0.06
      P-value <0.001 <0.001
      P-value for interaction 0.804
      P-value for intervention 0.288
      PCO2 Immediately after donation 49.60±8.33 57.25±10.63 0.003
      4 Hours after donation 54.81±7.79 62.23±7.61 0.001
      28 Days after donation 27.31±11.19 27.72±9.8 0.883
      P-value <0.001 <0.001
      P-value for interaction 0.594
      P-value for intervention 0.239
      pO2 Immediately after donation 190.82±124.57 68.94±18.62 <0.001
      4 Hours after donation 131.60±61.84 71.38±20.31 <0.001
      28 Days after donation 237.82±36.90 243.09±31.10 0.568
      P-value <0.001 <0.001
      P-value for interaction 0.033
      P-value for intervention 0.393
      HCO3 Immediately after donation 16.83±2.84 17.01±2.21 0.785
      4 Hours after donation 15.40±1.66 16.34±1.38 0.021
      28 Days after donation 2.09±1.74 1.98±1.66 0.800
      P-value <0.001 <0.001
      P-value for interaction 0.500
      P-value for intervention 0.878
      Na Immediately after donation 146.75±5.16 148.51±3.66 0.135
      4 Hours after donation 149.52±4.03 150.84±4.00 0.210
      28 Days after donation 140.14±6.75 142.77±7.75 0.168
      P-value <0.001 <0.001
      P-value for interaction 0.153
      P-value for intervention 0.102
      K Immediately after donation 3.22±1.76 2.85±0.50 0.269
      4 Hours after donation 2.63±0.46 2.73±0.59 0.494
      28 Days after donation 17.23±2.39 18.06±2.31 0.178
      p-value <0.001 <0.001
      p-value for interaction 0.352
      p-value for intervention 0.281
      Glucose Immediately after donation 431.53±132.08 513.67±90.18 0.007
      4 Hours after donation 416.63±123.37 446.17±123.33 0.602
      28 Days after donation 223.63±68.40 235.43±60.97 0.538
      p-value <0.001 <0.001
      p-value for interaction 0.503
      p-value for intervention 0.509
      Lactate Immediately after donation 11.39±3.66 12.46±3.05 0.224
      4 Hours after donation 21.44±4.29 19.34±4.14 0.059
      28 Days after donation 175.03±15.26 173.40±13.73 0.665
      P-value <0.001 <0.001
      P-value for interaction 0.364
      P-value for intervention 0.543
      RBC Immediately after donation 3.92±0.53 4.11±0.45 0.143
      4 Hours after donation 3.95±0.75 3.95±0.95 0.986
      28 Days after donation 3.24±0.77 3.47±0.69 0.233
      Immediately after donation <0.001 <0.001
      P-value for interaction 0.210
      P-value for intervention 0.448
      WBC Immediately after donation 5.45±1.39 5.31±1.06 0.643
      4 Hours after donation 5.24±1.40 5.74±1.53 0.189
      28 Days after donation 4.69±1.48 4.84±1.24 0.665
      P-value 0.001 0.014
      P-value for interaction 0.095
      P-value for intervention 0.783
      Hemoglobin Immediately after donation 12.12±1.66 12.52±1.50 0.327
      4 Hours after donation 11.99±2.13 11.83±2.11 0.762
      28 Days after donation 9.96±2.39 10.30±2.20 0.569
      Immediately after donation <0.001 <0.001
      P-value for interaction 0.703
      P-value for intervention 0.867
      Hematocrit Immediately after donation 35.82±4.50 37.84±3.31 0.055
      4 Hours after donation 35.89±6.17 35.67±5.47 0.886
      28 Days after donation 34.61±7.82 35.95±7.30 0.497
      Immediately after donation 0.445 0.122
      P-value for interaction 0.413
      P-value for intervention 0.394
      MCV Before CABG 90.67±3.96 92.16±4.87 0.196
      End of CABG 90.12±6.53 91.58±4.44 0.313
      28 Days after CABG 103.14±10.21 104.15±11.66 0.722
      P-value <0.001 <0.001
      P-value for interaction 0.105
      P-value for intervention 0.775
      MCH Immediately after donation 30.79±1.79 30.75±1.88 0.926
      4 hours after donation 31.05±1.71 31.03±2.15 0.971
      28 Days after donation 30.88±1.84 30.67±1.71 0.651
      P-value 0.476 0.109
      P-value for interaction 0.650
      P-value for intervention 0.305
      MCHC Immediately after donation 33.74±2.03 33.53±1.89 0.679
      4 Hours after donation 33.85±1.88 33.94±1.90 0.848
      28 Days after donation 29.85±2.27 29.74±2.67 0.864
      P-value <0.001 <0.001
      P-value for interaction 0.211
      P-value for intervention 0.388
      Platelet Immediately after donation 190.30±45.67 170.37±41.11 0.081
      4 Hours after donation 171.67±40.42 158.07±39.57 0.193
      28 Days after donation 154.17±89.87 139.17±53.04 0.434
      P-value 0.045 0.004
      P-value for interaction 0.022
      P-value for intervention 0.326
      Urea Immediately after donation 31.50±8.71 32.51±9.60 0.672
      4 Hours after donation 32.71±9.38 32.61±9.02 0.968
      28 Days after donation 36.73±11.22 37.48±9.92 0.786
      P-value <0.001
      P-value for interaction 0.523
      P-value for intervention 0.192
      Creatinine Immediately after donation 1.03±0.20 1.10±0.21 0.232
      4 Hours after donation 1.09±0.18 1.16±0.22 0.186
      28 Days after donation 1.18±0.27 1.22±0.28 0.623
      P-value <0.001 <0.001
      P-value for interaction 0.594
      P-value for intervention 0.092
      LDH Immediately after donation 228.20±70.37 245.60±68.50 0.336
      4 Hours after donation 240.23±95.93 259.07±92.10 0.441
      28 Days after donation 1,306.37±352.06 1,230.08±396.83 0.434
      P-value <0.001 <0.001
      P-value for interaction 0.133
      P-value for intervention 0.818
      Immediately after donation Immediately after donation 3.33±0.56 3.51±0.46 0.170
      4 Hours after donation 3.23±0.52 3.36±0.56 0.350
      28 Days after donation 3.32±0.45 3.48±0.44 0.147
      P-value 0.363 0.010
      P-value for interaction 0.579
      P-value for intervention 0.443
      Table 1. Comparison of demographic variables between groups

      Values are presented as mean±standard deviation or number (%).

      Table 2. Comparison between and within blood groups

      PCO2: partial pressure of carbon dioxide; PO2: partial pressure of oxygen; HCO3: bicarbonate; Na: sodium; K: potassium; RBC: red blood cell; WBC: white blood cell; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; LDH: lactate dehydrogenase.

      Table 1.

      Table 2.

      ACC : Acute and Critical Care
      © The Korean Society of Critical Care Medicine.
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