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Original Article
Basic science and research
Feasibility study of incident dark-field video microscope for measuring microcirculatory variables in the mouse dorsal skinfold chamber model
Christine Kang, Ah-Reum Cho, Hyeon Jeong Lee, Hyae Jin Kim, Eun-Jung Kim, Soeun Jeo, Jeong-Min Hong, Daehoan Moon
Acute Crit Care. 2021;36(1):29-36.   Published online February 26, 2021
DOI: https://doi.org/10.4266/acc.2020.00969
  • 6,074 View
  • 141 Download
  • 1 Web of Science
  • 1 Crossref
AbstractAbstract PDFSupplementary Material
Background
Despite the importance of microcirculation in organ function, monitoring microcirculation is not a routine practice. With developments in microscopic technology, incident dark field (IDF) microscopy (Cytocam) has allowed visualization of the microcirculation. Dorsal skinfold chamber (DSC) mouse model has been used to investigate microcirculation physiology. By employing Cytocam-IDF imaging with DSC model to assess microcirculatory alteration in lipopolysaccharide (LPS)-induced endotoxemia, we attempted to validate availability of Cytocam-IDF imaging of microcirculation.
Methods
DSC was implanted in eight BALB/c mice for each group; control and sepsis. Both groups were given 72 hours to recover from surgery. The sepsis group had an additional 24-hour period of recovery post-LPS injection (4 mg/kg). Subsequently, a video of the microcirculation was recorded using Cytocam. Data on microcirculatory variables were obtained. Electron microscopy was implemented using lanthanum fixation to detect endothelial glycocalyx degradation.
Results
The microcirculatory flow index was significantly lower (control, 2.8±0.3; sepsis, 2.1±0.8; P=0.033) and heterogeneity index was considerably higher (control, 0.10±0.15; sepsis, 0.53±0.48; P=0.044) in the sepsis group than in the control group. Electron microscopy revealed glycocalyx demolishment in the sepsis group.
Conclusions
Cytocam showed reliable ability for observing changes in the microcirculation under septic conditions in the DSC model. The convenience and good imaging quality and the automatic analysis software available for Cytocam-IDF imaging, along with the ability to perform real-time in vivo experiments in the DSC model, are expected to be helpful in future microcirculation investigations.

Citations

Citations to this article as recorded by  
  • Sedation with propofol and isoflurane differs in terms of microcirculatory parameters: A randomized animal study using dorsal skinfold chamber mouse model
    Christine Kang, Ah-Reum Cho, Haekyu Kim, Jae-Young Kwon, Hyeon Jeong Lee, Eunsoo Kim
    Microvascular Research.2024; 153: 104655.     CrossRef
Review Article
Nutrition
Fluid management in perioperative and critically ill patients
Dongho Kang, Kyung Yeon Yoo
Acute Crit Care. 2019;34(4):235-245.   Published online November 29, 2019
DOI: https://doi.org/10.4266/acc.2019.00717
  • 11,508 View
  • 900 Download
  • 12 Web of Science
  • 11 Crossref
AbstractAbstract PDF
Fluid therapy to restore and/or maintain tissue perfusion may affect patient outcomes in perioperative, emergency, and intensive care. Kinetic analyses and outcome-oriented studies have provided more insight into fluid management. Crystalloids are slowly distributed to the interstitial space, and the efficiency (proportion of infused fluid retained in the bloodstream) is 50%−75% as long as infusion continues and may increase up to 100% when the arterial pressure has decreased. Elimination of the infused fluid during general anesthesia and surgery is very slow, amounting to only 10%–20% compared with that in conscious patients. When the endothelial glycocalyx layer is degraded in sepsis or trauma-induced systemic inflammation, turnover of colloids and crystalloids is accelerated and the efficiency is reduced, which may lead to tissue edema, inflammation, poor wound healing, and organ dysfunction. Balanced crystalloids are pragmatic initial resuscitation fluids and improve patient outcomes compared to saline (0.9% sodium chloride). Albumin may be beneficial, but other synthetic colloids appear to increase the risk of acute kidney injury and death among patients in the intensive care unit. Fluid kinetics is likely to change based on patient physiological conditions (e.g., general anesthesia, surgery, stress, dehydration, blood pressure, or inflammation) and fluid types. To maximize efficacy and minimize iatrogenic side effects, fluids should be prescribed based on individual patient factors, disease states, and other treatment remedies.

Citations

Citations to this article as recorded by  
  • The incidence and risk factors of proximal lower extremity deep vein thrombosis without pharmacologic prophylaxis in critically ill surgical Taiwanese patients: A prospective study
    Ting-Lung Lin, Wen-Hao Liu, Wei-Hung Lai, Ying-Ju Chen, Po-Hsun Chang, I-Ling Chen, Wei-Feng Li, Yueh-Wei Liu, Eric J Ley, Chih-Chi Wang
    Journal of the Intensive Care Society.2024; 25(2): 140.     CrossRef
  • Pulse pressure variation guided goal-direct fluid therapy decreases postoperative complications in elderly patients undergoing laparoscopic radical resection of colorectal cancer: a randomized controlled trial
    Qiu-Rong Wu, Zi-Zuo Zhao, Ke-Ming Fan, Hui-Ting Cheng, Bin Wang
    International Journal of Colorectal Disease.2024;[Epub]     CrossRef
  • Prevalence and associated factors of postoperative orthostatic intolerance at University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia, 2022: cross sectional study
    Negesse Zurbachew Gobezie, Nigussie Simeneh Endalew, Hailu Yimer Tawuye, Habtu Adane Aytolign
    BMC Surgery.2023;[Epub]     CrossRef
  • Remimazolam and serious adverse events
    Sander Kempenaers, Tom G. Hansen, Marc Van de Velde
    European Journal of Anaesthesiology.2023; 40(11): 841.     CrossRef
  • Meropenem pharmacokinetics in critically ill patients with or without burn treated with or without continuous veno‐venous haemofiltration
    Daniel J. Selig, Kevin S. Akers, Kevin K. Chung, Kaitlin A. Pruskowski, Jeffrey R. Livezey, Elaine D. Por
    British Journal of Clinical Pharmacology.2022; 88(5): 2156.     CrossRef
  • Does perioperative fluid management affect the development of postoperative complications in major gastrointestinal tract surgery? A retrospective cohort study
    Mehmet Mustafa ALTINTAŞ, Kemal Tolga SARAÇOĞLU, Aytaç Emre KOCAOĞLU, Fırat MÜLKÜT, Ayten SARACOĞLU, Selçuk KAYA, Ayhan ÇEVİK
    Journal of Surgery and Medicine.2022; 6(2): 90.     CrossRef
  • A Porcine Sepsis Model With Numerical Scoring for Early Prediction of Severity
    Attila Rutai, Bettina Zsikai, Szabolcs Péter Tallósy, Dániel Érces, Lajos Bizánc, László Juhász, Marietta Zita Poles, József Sóki, Zain Baaity, Roland Fejes, Gabriella Varga, Imre Földesi, Katalin Burián, Andrea Szabó, Mihály Boros, József Kaszaki
    Frontiers in Medicine.2022;[Epub]     CrossRef
  • Risk Factors for and Outcomes Associated With Peri-Intubation Hypoxemia: A Multicenter Prospective Cohort Study
    Nathan J. Smischney, Ashish K. Khanna, Ernesto Brauer, Lee E. Morrow, Uchenna R. Ofoma, David A. Kaufman, Ayan Sen, Chakradhar Venkata, Peter Morris, Vikas Bansal
    Journal of Intensive Care Medicine.2021; 36(12): 1466.     CrossRef
  • Inflammatory response, fluid balance and outcome in emergency high‐risk abdominal surgery
    Mirjana Cihoric, Henrik Kehlet, Morten L. Lauritsen, Jakob Højlund, Katrine Kanstrup, Nicolai B. Foss
    Acta Anaesthesiologica Scandinavica.2021; 65(6): 730.     CrossRef
  • A review on the physiological and pathophysiological role of endothelial glycocalyx
    Huan‐qiu Liu, Ji Li, Cheng‐luan Xuan, Hai‐chun Ma
    Journal of Biochemical and Molecular Toxicology.2020;[Epub]     CrossRef
  • Science‐in‐brief: The role of the glycocalyx in critically ill patients with reference to the horse
    Bettina Dunkel
    Equine Veterinary Journal.2020; 52(6): 790.     CrossRef

ACC : Acute and Critical Care