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1 "Young-Min Hyun"
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Basic science and research
Two-photon intravital imaging of leukocyte migration during inflammation in the respiratory system
Young Min Kim, Soi Jeong, Young Ho Choe, Young-Min Hyun
Acute Crit Care. 2019;34(2):101-107.   Published online May 31, 2019
DOI: https://doi.org/10.4266/acc.2019.00542
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AbstractAbstract PDF
Two-photon intravital imaging is a powerful method by which researchers are able to directly observe biological phenomena in live organisms. Researchers in various biomedical research fields have applied two-photon imaging to a variety of target organs by utilizing this technology’s ability to penetrate to significant depths with minimal phototoxicity. The mouse respiratory system in inflammation models is a good example, as two-photon intravital imaging can provide insights as to how the immune system is activated in response to inflammation within the respiratory system. Inflammation models can be generated via influenza viral, bacterial, or lipopolysaccharide injection. To exteriorize the lungs or trachea, thoracotomy or tracheotomy is performed, respectively; the appropriate combination of inflammation induction and organ exposure is selected depending on the study purpose. On the other hand, visualizing the movement of leukocytes is also an important component; to this end, immune cell populations of interest are either labeled via the genetic attachment of fluorescent proteins or stained with antibodies or dyes. With the proper selection of methods at each step, twophoton intravital imaging can yield visual evidence regarding immune responses to inflammation.

Citations

Citations to this article as recorded by  
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    Trends in Immunology.2024;[Epub]     CrossRef
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  • Intravital Imaging of Pulmonary Immune Response in Inflammation and Infection
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    Frontiers in Cell and Developmental Biology.2021;[Epub]     CrossRef
  • Probe-based intravital microscopy: filling the gap between in vivo imaging and tissue sample microscopy in basic research and clinical applications
    Katrien Van Dyck, Eliane Vanhoffelen, Jonas Yserbyt, Patrick Van Dijck, Marco Erreni, Sophie Hernot, Greetje Vande Velde
    Journal of Physics: Photonics.2021; 3(3): 032003.     CrossRef

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